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KMID : 0360220150560010104
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Journal of the Korean Ophthalmological Society
2015 Volume.56 No. 1 p.104 ~ p.108
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Effect of Benzalkonium, Mitomycin-C and Dexamethasone on Stress in Trabecular Meshwork Cells
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Kim Byung-Moon
Kim Jae-Woo
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Abstract
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Purpose: To investigate the effects of benzalkonium chloride (BAC), mitomycin C (MMC) and dexamethasone (DEX) on cellular stress in cultured human trabecular meshwork cell (HTMC) monolayers.
Methods: HTMCs were cultured in the inner Transwell chamber until confluence and then were exposed to BAC, MMC or DEX for 6 hours. The carboxyfluorescein permeability through the HTMC monolayer was measured using a spectrofluorometer at 532 nm after 2 hours in the outer chamber. The 3-[4, 5 -dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) assay was used to evaluate cellular viabilities.
Results: The carboxyfluorescein permeability through the HTMC monolayer increased and cell survival decreased with 0.002% BAC (p < 0.05). Increased permeability without decreasing cell survival occurred with 0.05 μg/mL MMC. No effect on the permeability or cell survival was observed at 0.1 or 1.0 μm DEX (p > 0.05).
Conclusions: BAC and MMC induced cellular toxicity and stress at lower concentrations but did not affect survival of cultured HTMCs.
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KEYWORD
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Benzalkonium chloride, Carboxyfluorescein, Dexamethasone, Mitomycin C, Trabecular meshwork cells
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